BRCA1 anticorps (AA 341-748)

N° du produit ABIN7468063

L’anticorps Souris Monoclonal anti-BRCA1 a été validé pour WB, IHC, IF, IHC (p), IP, ICC et ChIP. Il convient pour détecter BRCA1 dans des échantillons de Humain.

Aperçu rapide pour BRCA1 anticorps (AA 341-748) (ABIN7468063)

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BRCA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Classe de qualité: KO Validated

Clone: 6B4

Détail du produit anti-BRCA1 anticorps

Épitope: AA 341-748

Specificité: This antibody recognizes BRCA1, a 220- kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.

Réactivité croisée: Humain

Purification: Protein G purified

Immunogène: BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T , MCF7 , U2OS , HeLa Validation: KO/KD

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.75 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur BRCA1

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: BRCA1 DNA repair associated

Sujet:

Synonyms: BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Background: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Poids moléculaire: 208 kDa

ID gène: 672

UniProt: P38398

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus